Reliability of mouse embryo assay on quality control of human assisted reproductive technologies
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How to Cite

Junzhao. (2017). Reliability of mouse embryo assay on quality control of human assisted reproductive technologies. American Journal of Translational Medicine, 1(3), 134–147. Retrieved from https://ajtm.journals.publicknowledgeproject.org/index.php/ajtm/article/view/890

Abstract

The currently used standard mouse embryo assay (MEA), which is commonly defined as the blastocyst rate (BR) produced from one-cell hybrid embryo in vitro, has been used for quality control (QC) of the reagents, plastic consumables and equipment in human assisted reproductive technologies (ART) clinic. There have been concerned whether these MEA results are suitable to be clinically relevant as numerous data are obviously missing and known to be largely factorial. Ideal MEA standards should include all possible variations and all check-end levels under the most stressful conditions. Systematic analysis of the factors on MEA indicates that counting of the cells in multiple developmental time-points and differential number of the cells in the inner cell mass (ICM) and trophectoderm (TE) together with time-lapse morpho-kinetic in outbred strain mice should be preferably added to current industry standard MEA. More stressful conditions including IVM/IVF procedures, oxygen tension, culture embryo density, frozen embryo and exogenous toxic agents (such as Triton x 100) can be further used for improving the sensitivity of MEA. Current ART is urgently requiring more sensitive MEA to ensure QC of treatment of infertility. (Am J Transl Med 2017. 1:134-147)

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