Abstract
Allergies to mango (Mangifera indica L.) are frequently encountered in clinical practice. However, only two allergens have been identified from mango so far. Here the protein crude extracts of mangos were resolved by using 1-D sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by western blot. A predominant reactive band with a molecular mass of 30 kDa was analyzed by MS/MS mass spectrometry. The band contained protein fragments which exhibited significant homology to mango chitinase (GenBank Accession: ACD69683.1). The cDNA of the chitinase was synthesized and cloned into a pET-28a plasmid and expressed as a recombinant His-tagged protein. The reactivity of the recombinant protein toward mango-allergic patients was assayed by using western blot. The linear IgE epitopes of the chitinase were then analyzed by spanning peptides. The mango chitinase reacted with IgE of 9 out of 13 sera (69%) of pediatric patients with mango allergy. Only one peptide (8-28) which contained the chitin-binding domain possessed IgE reactivity. (Am J Transl Med 2018. 2:68-76)