Development of a cuproptosis-related prognostic signature and associated regulatory axis in colon cancer
PDF

Keywords

colon cancer
cuproptosis
prognostic model
tumor immune microenvironment
FDX1

Categories

How to Cite

Yin, X., hu, zhiqian, Li, X., xu, kai, wei, shuxun, jin, taojun, … zhang, wei. (2023). Development of a cuproptosis-related prognostic signature and associated regulatory axis in colon cancer. American Journal of Translational Medicine, 7(2), 110–121. Retrieved from https://ajtm.journals.publicknowledgeproject.org/index.php/ajtm/article/view/2762

Abstract

OBJECTIVES: Based on the TCGA and GEO databases, we constructed a cuproptosis-related gene signature to predict colon cancer prognosis. Furthermore, we explored the role of cuproptosis regulators in the development of colon cancer. METHODS: In the discovery set from the TCGA-COAD cohort (n = 452), cuproptosis regulators were incorporated in a LASSO-COX regression to construct our signature. The predictive ability was validated in the GEO cohort (n = 562). Subsequently, the immune microenvironment was compared between high- and low-risk groups according to risk score. The role of FDX1 during colon cancer was also investigated. RESULTS: A four-gene signature was successfully constructed. This signature could discriminate colon cancer with different prognoses both in the discovery (P < 0.001) and validation sets (P = 0.015). Immune infiltration analysis revealed that tumors in the high-risk group had more infiltrated CD8+ T and activated NK cells (both P < 0.001), while low-risk tumors had more infiltrated CD4+ T (P < 0.001) and plasma cells (P < 0.01). Moreover, the hsa-miR-9-3p/FDX1 pathway was found in colon cancer. CONCLUSION: We constructed a cuproptosis-related gene signature in colon cancer; its performance was robust in the discovery and validation cohorts. The relationship between FDX1 and tumorigenesis in colon cancer was also elucidated.

PDF